Use of frozen chick and duck embryo cells for plaque assays of arboviruses and rickettsiae |
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| Authors: | A T McManus D R Parker R H Henyon J P Kondig G A Eddy |
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| Affiliation: | 2. U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Md 21701, U.S.A.;1. Institute for Human Infections and Immunity, Center for Tropical Diseases, and Department of Pathology, University of Texas Medical Branch, 301 University Blvd., Galveston, TX 77555, USA |
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| Abstract: | Conditions for preparation, freezing, storing and plating of chick and duck embryo cell slurries are described. Comparisons of plaque assay sensitivity for fresh and previously frozen cells with Eastern equine encephalitis, Western equine encephalitis, Venezuelan equine encephalitis and Semliki Forest virus showed, for each virus, that the four classes of cells gave similar titres. Vesicular stomatis virus assayed equivalently in fresh and previously frozen chick cells, but was lower in duck embryo cells. Rickettsia rickettsii showed equal plaquing titre on chick and duck embryo cells. |
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