水稻雄蕊成熟导管ATPase的超微结构定位

采用ATPase定位方法研究了水稻农垦58s-SD(可育花药)单核边位至三核期的花丝和药隔成熟导管。单核边位期花丝和药隔成熟导管内无ATPase活性;二核期花丝导管内出现大量的ATPase,导管周围的薄壁细胞中有1-2个优先解体,在导管无次生壁的部位,解体薄壁细胞靠近导管处的质膜ATPase不活跃;二核期药隔导管内也有大量的ATPase活性物,但导管周围细胞解体晚于花丝;三核期花丝和药隔导管内也观察到大量的具或不具ATPase活性的物质。以上结果暗示成熟导管内ATPase活性物(一部分)可能来源于花丝解体细胞。

ULTRASTRUCTURAL LOCALIZATION OF ATPase IN MATURE TRACHEARY ELEMENTS OF RICE (Oryza sativa L. ) STAMEN

The development of mature tracheary elements (TEs) of rice(Oryza sativa L. cv. Nongkeng 58s-SD) stamen from big-vacuole to trinucleate pollen stage was studied with lead phosphate - deposition technique. At big-vacuole pollen stage, mature TEs, both in filament and connective, were short of ATPase activity. However, ATPase activity appeared at binucleate pollen stage; in filament, one or two vascular bundle cell (s) next to TE degenerated earlier than the rest; and the plasma membrane of degenerating cell (DC) always exhibited high ATPase activity on condition that the adjoining TE was of fully stretched second wall; when the second wall only turned up far off DC, the plasma membrane of DC still showed high ATPase activity except on borderline between DC and TE; in connective at the same stage, TEs also exhibited high ATPase activity, but the vascular bundle cell(s) adjoining TEs degenerated much later than their counterparts in filament. At trinucleate pollen stage, materials, either with or without ATPase activity, were observed both in filament and connective TEs. The above results indicated that (some of the) materials in mature TEs come from filament DC.