Protection induced in BALB/c mice by the high-molecular-mass (hMM) fraction of <Emphasis Type="Italic">Paracoccidioides brasiliensis</Emphasis> |
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Authors: | Wander R Pavanelli Mari S Kaminami Juliana R Geres Ayako Sano Mario A Ono I C C Camargo Eiko N Itano |
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Institution: | 1.Funda??o Araucária,Curitiba,Brazil;2.Department of Pathology Science,CCB, State University of Londrina,Londrina,Brazil;3.Coordena??o de Aperfei?oamento de Pessoal de Nível Superior (CAPES),Brasilia,Brazil;4.Research Center of Pathogenic Fungi and Microbial Toxicoses,Chiba University,Chiba,Japan;5.Department of Biology Science,Paulista State University,Assis,Brazil |
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Abstract: | Paracoccidioidomycosis (PCM) is a granulomatous disease caused by a dimorphic fungus, Paracoccidioides brasiliensis. The present study investigated the protective activity of the P. brasiliensis high-molecular-mass (hMM) fraction (~380 kDa) in experimental murine PCM. In the first step, lymphocyte proliferation and
production of IFNγ (but not IL-4) were observed in “in vitro” spleen cells (from female BALB/c mice infected (i.v.) with P. brasiliensis) that were stimulated with hMM fractions. In the second step, female BALB/c mice were previously immunized (s.c.) with hMM
fraction (25 μg/protein = F-25 and 50 μg/protein = F-50), and the colony-forming units (CFU) of the lung and spleen, the histopathological
characteristics of the granulomatous lesions, and plasmatic gp43 soluble antigens and anti-hMM IgG levels were analyzed at
28 and 56 days after infection. The lung and liver CFU were lower in mice previously immunized with the hMM fraction (P < 0.05). The granulomatous lesions revealed a greater degree of compaction and organization, with no dissemination of the
fungus to other organs. Lower soluble antigen levels (P < 0.05) and higher IgG anti-hMM fraction (P < 0.05) were observed in immunized groups. The results for CFU, histopathology and antigenemia suggest that the hMM fraction
has a protective effect in experimental paracoccidioidomycosis in BALB/c mice. |
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Keywords: | antigenemia CFU granuloma Paracoccidioides brasiliensis paracoccidioidomycosis protection |
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