| HCMV感染抑制人海马神经干细胞分化 |
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| 引用本文： | 李玲,王斌,李鹏,白志强,王海涛,宋旭霞,丁守怡.HCMV感染抑制人海马神经干细胞分化[J].病毒学报,2009,25(3):196-201. |
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| 作者姓名： | 李玲 王斌 李鹏 白志强 王海涛 宋旭霞 丁守怡 |
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| 作者单位： | 青岛大学,医学院,组胚教研室,青岛,266071;青岛大学,医学院,微生物教研室,青岛,266071 |
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| 基金项目： | 国家自然基金资助项目(30770105);;青岛市科技计划项目(08-1-3-30-jch);;泰山学者建设工程专项经费资助 |
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| 摘 要： | 研究HCMV感染对体外培养的人海马源性神经干细胞(Neural stem cells,NSCs)分化的影响。体外分离、培养人海马NSCs,应用免疫荧光方法检测其NSCs标记物-巢蛋白(Nestin)的表达。10%胎牛血清诱导NSCs贴壁分化,同时用MOI为5的HCMV AD169株感染NSCs,7d后使用激光共聚焦显微镜免疫荧光方法检测Nestin、神经胶质纤维酸性蛋白(GFAP)和HCMV即刻早期蛋白(IE)的表达,计算阳性细胞比率。本实验所培养的细胞(4～6代)95±8%表达Nestin;分化诱导7d后,感染组86±12%细胞表达IE,未感染组和感染组Nestin阳性率分别为50±19%和93±10%(t=6.03,P<0.01),GFAP阳性细胞率分别为81±11%和55±17%(t=3.77,P<0.01)。以上结果表明分化过程中的NSCs是HCMV的容许细胞;HCMV感染可以抑制NSCs的分化。
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| 关 键 词： | 人巨细胞病毒 神经干细胞 海马 细胞分化 巢蛋白 |
Human Cytomegalovirus Inhibits the Differentiation of Human Hippocampus Neural Stem Cells |
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| LI ling,WANG Bin,LI Peng,BAI Zhi-qiang,WANG Hai-tao,SONG Xu-Xia,DING Shou-yi.Human Cytomegalovirus Inhibits the Differentiation of Human Hippocampus Neural Stem Cells[J].Chinese Journal of Virology,2009,25(3):196-201. |
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| Authors: | LI ling WANG Bin LI Peng BAI Zhi-qiang WANG Hai-tao SONG Xu-Xia DING Shou-yi |
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| Institution: | 1.Department of Histology and Embryology;Medical College of Qingdao University;Shandong Qingdao 266071;2.Department of Microbiology;Medical College of Qingdao University Shandong Qingdao 266071;China |
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| Abstract: | The objective of present study is to investigate the effect of human cytomegalovirus(HCMV) infection on human hippocampus neural stem cells NSCs differentiation in vitro, Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. Immunofluorescence method was used to detect the expression of NSCs marker-Nestin within these cells. Cultured in 10%FBS, NSCs began to differentiate. On the onset of the differentiation, HCMV AD169(MOI=5) was added into the d... |
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| Keywords: | human cytomegalovirus neural stem cells cell differentiation hippocampus nestin |
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