Ultrahistochemical study on the ruthenium red surface staining |
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Authors: | Pierre R Blanquet |
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Institution: | (1) Institut de Recherches sur le Cancer et Unité 124 de l'Institut National de la Santé et la Reserche Médicale, B.P. 3567, F-59020 Lille Cedex, France |
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Abstract: | Summary The cell coat picture effect which is usually obtained with the conventional RR method, that is with the RR/OsO4 coupled reaction, is investigated. In this first paper, each of conceivable events which might take place between RR, OsO4 and cell surface membrane is discussed or studied. Various tests are carried out on ascites Ehrlich carcinoma cells and Zajdela ascites hepatoma cells treated with numerous chemical reagents, as also on a few pure proteins. The set of data supports the concept that the staining pattern is due to the combination in surface membranes of RR with a colloidal-like form of OsO2. The latter might occur during the formation of stable cyclic osmic acid diesters between OsO4 and membrane unsaturated lipids. A possibility by which the resulting marker is though also to be in a colloidal-like state is put forward. A next report will deal with this problem. |
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