Effects of temperature,photoperiod and culture vessel size on adventitious shoot production of in vitro propagated <Emphasis Type="Italic">Huernia hystrix</Emphasis> |
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Authors: | S O Amoo J F Finnie J Van Staden |
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Institution: | (1) Research Centre for Plant Growth and Development, School of Biological and Conservation Sciences, University of KwaZulu-Natal Pietermaritzburg, Private Bag X01, Scottsville, 3209, South Africa; |
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Abstract: | High production costs due to low growth rate in vitro and high labour costs are among factors limiting commercial application
of micropropagation techniques. The low growth rate could be due to unfavourable or sub-optimal environmental and chemical
conditions of the cultures. The effects of temperature, photoperiod and culture vessel size were investigated on adventitious
shoot production of Huernia hystrix. There were significant increases in shoot proliferation with increased temperature in cultures maintained under a 16 h photoperiod.
Slow growth observed at low temperatures (15 and 20°C) offers a potential strategy for cost-effective in vitro storage of
H. hystrix germplasm. The maximum adventitious shoots produced per explant and percentage of explants producing shoots (4.2 ± 0.74 and
94% respectively) were observed in cultures maintained at 35°C, the optimum temperature for photosynthesis in plants possessing
crassulacean acid metabolism (CAM). The nocturnal accumulation of organic acids in cultures incubated under a 16 h photoperiod
further suggest the presence of CAM in this species. On the other hand, cultures kept under continuous light appear to shift
to a C-3 photosynthetic pathway. There was a significant decrease in fresh weight of adventitious shoots regenerated per explant
as temperature increased. The use of larger culture vessels further increased the shoot proliferation to 5.6 shoots per explant
with a potential production of 3,429 shoots per m2 in the growth room compared to 2,750 shoots per m2 using culture tubes. |
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