|
|||||
|
|
Molecular characterization of a fungal gene paralogue of the penicillin penDE gene of Penicillium chrysogenum |
|
| Authors: | Carlos García-Estrada Inmaculada Vaca Ricardo V Ullán Marco A van den Berg Roel AL Bovenberg Juan Francisco Martín |
| Affiliation: | 1. Instituto de Biotecnología (INBIOTEC), Parque Científico de León, Av. Real, 1, 24006, León, Spain 2. DSM Gist (624-0270), DSM Anti-Infectives, PO Box 425, 2600, Delft, AK, the Netherlands 3. área de Microbiología, Departamento de Biología Molecular, Facultad de CC Biológicas y Ambientales, Universidad de León, Campus de Vegazana s/n, 24071, León, Spain |
| Abstract: | BackgroundPenicillium chrysogenum converts isopenicillin N (IPN) into hydrophobic penicillins by means of the peroxisomal IPN acyltransferase (IAT), which is encoded by the penDE gene. In silico analysis of the P. chrysogenum genome revealed the presence of a gene, Pc13g09140, initially described as paralogue of the IAT-encoding penDE gene. We have termed this gene ial because it encodes a protein with high similarity to IAT (IAL for IAT-Like). We have conducted an investigation to characterize the ial gene and to determine the role of the IAL protein in the penicillin biosynthetic pathway. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |