Functional Rectification of the Newly Described African Henipavirus Fusion Glycoprotein (Gh-M74a) |
| |
| Authors: | Olivier Pernet Shannon Beaty Benhur Lee |
| |
| Affiliation: | aDepartment of Microbiology, Immunology and Molecular Genetics, University of California Los Angeles, Los Angeles, California, USA;bIcahn School of Medicine at Mount Sinai, New York, New York, USA |
| |
| Abstract: | Recent evidence identified multiple Henipavirus species in Africa distinct from those in Southeast Asia and Australia. The reported fusion glycoprotein (F) sequence of the African Gh-M74a strain (GhV-F) is likely incorrect: a single base pair deletion near the N terminus results in multiple aberrancies. Rectifying this by adding single nucleotide insertions results in a GhV-F that now possesses a signal peptide, is efficiently cell surface expressed, exhibits syncytium formation when coexpressed with GhV-G protein, and mediates pseudotyped viral particle entry. |
| |
| Keywords: | |
|
|
