Roles of Interleukin-9 in the Growth and Cholecystokinin-Induced Intracellular Calcium Signaling of Cultured Interstitial Cells of Cajal |
| |
| Authors: | Yaoyao Gong Lei Huang Wenfang Cheng Xueliang Li Jia Lu Lin Lin Xinmin Si |
| |
| Institution: | 1. Department of Gastroenterology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.; 2. Department of Pediatric Surgery, Nanjing Children’s Hospital Affiliated to Nanjing Medical University, Nanjing, China.; University of Nevada School of Medicine, United States of America, |
| |
| Abstract: | Interstitial cells of Cajal (ICC) are pacemaker cells in the gastrointestinal (GI) tract and loss of ICC is associated with many GI motility disorders. Previous studies have shown that ICC have the capacity to regenerate or restore, and several growth factors are critical to their growth, maintenance or regeneration. The present study aimed to investigate the roles of interleukin-9 (IL-9) in the growth, maintenance and pacemaker functions of cultured ICC. Here, we report that IL-9 promotes proliferation of ICC, and culturing ICC with IL-9 enhances cholecystokinin-8-induced Ca2+ transients, which is probably caused by facilitating maintenance of ICC functions under culture condition. We also show co-localizations of cholecystokinin-1 receptor and IL-9 receptor with c-kit by double-immunohistochemical labeling. In conclusion, IL-9 can promote ICC growth and help maintain ICC functions; IL-9 probably performs its functions via IL-9 receptors on ICC. |
| |
| Keywords: | |
|
|
