Identification and characterization of EST-SSRs and cpSSRs in endangered <Emphasis Type="Italic">Cycas hainanensis</Emphasis> |
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Authors: | Zheng-Feng Wang Wan-Hui Ye Hong-Lin Cao Zhong-Chao Li Shao-Lin Peng |
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Institution: | (1) Guangdong Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences, Xingke Route 723, TianHe, Guangzhou, 510650, P.R. China;(2) School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, P.R. China |
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Abstract: | We report on the identification and characterization of six EST-linked simple sequence repeats (EST-SSRs) and chloroplast
SSRs (cpSSRs) in endangered Cycas hainanensis. The number of alleles ranged from two to eight for EST-SSRs, two to three for cpSSRs. Observed and expected heterozygosities
ranged from 0.042 to 0.417 and 0.042 to 0.811 for EST-SSRs, respectively. Expected heterozygosities ranged from 0.156 to 0.457
for cpSSRs. All these markers gave successful cross-species amplification in C. fairylakea. These markers will allow analyses of the baseline genetic variability and population structure of C. hainanensis to provide strategies for effective conservation and management.
The experiments were carried out in South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, P.R. China. |
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Keywords: | Cycas hainanensis Expressed sequence tag Chloroplast Simple sequence repeat Primer |
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