Bismuth staining of a nucleolar protein |
| |
Authors: | Nicole Gas Genevive Inchausp Marie Claude Azum Barbara Stevens |
| |
Institution: | Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS, 31062 Toulouse Cedex, France |
| |
Abstract: | A major nucleolar protein in Chinese hamster ovary cells with a molecular weight (MW) of 100 kD has been found to stain selectively with the bismuth tartrate technique of Locke & Huie 19]. After glutaraldehyde fixation and bismuth staining of electrophoretic transfers of total nucleolar proteins separated by SDS-PAGE, a single band corresponding to the 100 kD protein is revealed. When the technique is applied to whole cells, small punctate regions of the nucleoli are strongly stained. At the ultrastructural level, bismuth selectively contrasts the fibrillar centers and the adjoining cords of the dense fibrillar component. The remainder of the dense fibrillar component is not stained. It is proposed that the high phosphorylation level of the 100 kD protein is responsible for its glutaraldehyde-insensitive bismuth staining. The concentration of this protein in certain localized regions of the nucleolus suggests that it plays a metabolic rather than a structural role. |
| |
Keywords: | To whom offprint requests should be sent |
本文献已被 ScienceDirect 等数据库收录! |