| 鲢鱼轻酶解肌球蛋白的cDNA克隆及结构解析 |
| |
| 引用本文： | 蒋旷,陶妍,王玲军,党静,谢晶.鲢鱼轻酶解肌球蛋白的cDNA克隆及结构解析[J].氨基酸和生物资源,2011,33(1):21-28,42. |
| |
| 作者姓名： | 蒋旷 陶妍 王玲军 党静 谢晶 |
| |
| 作者单位： | 上海海洋大学食品学院,上海,201306 |
| |
| 基金项目： | 上海市科委2008年度重点科技项目 |
| |
| 摘 要： | 与栖息温度相关的鲢鱼两种轻酶解肌球蛋白重链(light meromyosin,LMM)同工型(低温型,sc-w;高温型,sc-s)的氨基酸序列解析结果表明:sc-w与sc-s在LMM的氨基酸序列上显示91.8%的同源性,但与已经报道的草鱼(Ctenopharyngodon idella)低温型(gc10)有96.9%的...
|
| 关 键 词： | 鲢鱼 轻酶解肌球蛋白 cDNA克隆 氨基酸序列 |
cDNA Cloning and Structural Analysis of the Light Meromyosin from Silver Carp(Hypophthalmichthys molitrix) |
| |
| JIANG Kuang,TAO Yan,WANG Ling-jun,DANG Jing,XIE Jing.cDNA Cloning and Structural Analysis of the Light Meromyosin from Silver Carp(Hypophthalmichthys molitrix)[J].Amino Acids & Biotic Resources,2011,33(1):21-28,42. |
| |
| Authors: | JIANG Kuang TAO Yan WANG Ling-jun DANG Jing XIE Jing |
| |
| Institution: | JIANG Kuang,TAO Yan,WANG Ling-jun,DANG Jing and XIE Jing (College of Food Science and Technology,Shanghai Ocean University,Shanghai 201306,China) |
| |
| Abstract: | The analysis of primary structures for the two types of silver carp（Hypophthalmichthys molitrix） light meromyosin heavy chain（LMM） isoform（low temperature-type,sc-w;high temperature-type,sc-s） related to temperature showed that the primary structure of LMM produced a 91.8% identity between sc-w and sc-s.Whereas a high identity of 96.9% was found between sc-w and gc10 which was reported to be isolated from 10℃-acclimated grass carp.Comparing with the LMM isoforms from other animals,the isoform-specific differences for sc-w and gc10 were clearly observed in 21 amino acid residues.Furthermore,among these amino acid mutations,9 mutations occurred at the conserved residue sites.However,the ratio of hydrophobic residue to the total was higher for the sc-s than for the sc-w.These differences in the primary structures of the two LMM isoforms may result in the differences in gel-forming profiles of myosins from silver carp in different seasons. |
| |
| Keywords: | silver carp light meromyosin cDNA cloning amino acid sequence |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
