High frequency shoot regeneration from immature embryo explants of Hungarian vetch |
| |
Authors: | Sancak Cengiz Mirici Semra Özcan Sebahattin |
| |
Affiliation: | (1) G. B. Pant Institute of Himalayan Environment & Development, Kosi-Katarmal, Almora, 263643 (Uttaranchal), India |
| |
Abstract: | An in vitro propagation protocol has been developed from mature trees of Pittosporum napaulensis. The best bud proliferation (83.1%), shoot number (21 axillary shoots/ explant) and shoot length (5.5 cm) was achieved in Murashige and Skoog (MS) medium supplemented with 5.0 μM N−6 benzyladenine and 0.1 μM α- naphthalene acetic acid. Of the three cytokinins tested (N−6 benzyladenine, kinetin and thidiazuron), N−6 benzyladenine proved to be the best for shoot induction. Shoot regeneration potential varied among genotypes. Regenerated shoots rooted after 48 hours treatment on half-strength MS liquid medium supplemented with 20 μM indole-3-butyric acid. Rooted shoots transferred to 120 g (w/v) soilrite + sand + soil (1:1:1) mixture showed 70% survival. Twenty-one plantlets are growing well in green house conditions. This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | endemic medicinal micropropagation Pittosporum napaulensis rare |
本文献已被 SpringerLink 等数据库收录! |