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Molecular genetic and biochemical analysis of Brassica napus proliferating cell nuclear antigen function |
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| Authors: | Markley Nancy-Ann Young Dallan Laquel Patricia Castroviejo Michel Moloney Maurice M. |
| Affiliation: | (1) Department of Biological Sciences, University of Calgary, 2500 University Drive, NW, Calgary, Alberta, Canada, T2N 1N4;(2) Department of Medical Biochemistry, University of Calgary Health Sciences Center, Calgary, Alberta, Canada, T2N 4N1;(3) Institut de Biochimie et Génétique Cellulaires du Centre National de la Recherche Scientifique, Bordeaux, France |
| Abstract: | A cDNA encoding the proliferating cell nuclear antigen (PCNA) from Brassica napus (oilseed rape) was shown to complement the lethal deletion mutation in the PCNA gene ( POL30) of Saccharomyces cerevisiae. We provide unequivocal evidence that the B. napus PCNA can perform all the essential functions of the yeast PCNA in DNA replication, although some species-specific differences may exist. In addition, the B. napus PCNA expressed as a fusion polypeptide with glutathione S-transferase (GST) was shown to stimulate the activity and processivity of two  -like DNA polymerases from wheat in vitro. These experiments provide direct biochemical evidence that the B. napus PCNA may function as an auxiliary factor in plant cell DNA replication. |
| Keywords: | Brassica napus complementation DNA polymerase |
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