Biodiesel production from waste cooking oil by immobilized lipase on superparamagnetic Fe3O4 hollow sub-microspheres |
| |
Authors: | Changxia Liu Jinlei Yuan Huafeng Gao |
| |
Institution: | College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, PR China |
| |
Abstract: | Superparamagnetic Fe3O4 hollow sub-microspheres (FHSM) with strong response to an external magnet were prepared via a solvothermal method, followed by acid etching. Lipase from Candida sp. 99–125 was directly immobilized onto the amino-functional FHSM by simple adsorption, without glutaraldehyde linkage. The immobilized lipase was used to catalyze the esterification/transesterification of waste cooking oil with methanol to produce fatty acid methyl ester (FAME), a major source of biodiesel. FAME yield exceeded 93.4% over a wide range of temperatures from 10 to 40?°C. Notably, stability was clearly improved at the lower temperatures, in particular, giving a FAME yield of 89.6% after eight cycles of use at 10?°C. |
| |
Keywords: | Biodiesel Fe3O4 hollow sub-microspheres immobilized lipase reusability waste cooking oil |
|
|