Microbial hydroxylation of 17β-estradiol by Penicillium brevicompactum |
| |
| Authors: | Lihong Shan Yang Li Yanjie Chen Minghui Yin Jiajia Huang Zhenzhong Zhang |
| |
| Institution: | 1. School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou, PR China,;2. Collaborative Innovation Center of New Drug Research and Safety Evaluation, Zhengzhou, PR China, and;3. The People’ Hospital of Jiaozuo City, Jiaozuo, PR China |
| |
| Abstract: | Microbial hydroxylation of 17β-estradiol (1) with Penicillium brevicompactum, a fungal species not used in biotransformation so far, yielded four metabolites: 1, 3, 5-estratriene-3, 15α-diol-17-one (2); 1, 3, 5-estratriene-3, 6α, 17β-triol (3); 1, 3, 5-estratriene-3, 15α, 17β-triol (4); and 1, 3, 5-estratriene-3, 6α, 15α-triol-17-one (5). All the products were determined by 1H NMR, 13C NMR, two-dimensional NMR, and HRMS techniques. Compounds 3, 4, and 5 are reported for the first time via microbial transformation, and 5 is a new compound as far as we know. Possible metabolic pathway of 17β-estradiol via Penicillium brevicompactum was also proposed. |
| |
| Keywords: | Biotransformation 17β-estradiol hydroxylation Penicillium brevicompactum |
|
|
