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Deciphering flux adjustments of engineered E. coli cells during fermentation with changing growth conditions
Institution:1. Department of Energy, Environmental, and Chemical Engineering, Washington University, St. Louis, MO, USA;2. Department of Chemical Engineering, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, USA;3. College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China;4. Department of Chemistry, Hamilton College, Clinton, NY, USA;5. Joint BioEnergy Institute, Emeryville, CA, USA;6. Department of Chemical & Biomolecular Engineering, University of California, Berkeley, CA, USA;7. Department of Bioengineering, University of California, Berkeley, CA, USA;8. Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA;9. California Institute of Quantitative Biosciences (QB3), University of California, Berkeley, CA, USA;10. Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kogle Allé, DK2970-Hørsholm, Denmark;11. Department of Biological Sciences, Rensselaer Polytechnic Institute, Troy, NY, USA;1. School of Biotechnology and Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China;2. Synergetic Innovation Center of Food Safety and Nutrition, 1800 Lihu Road, Wuxi, Jiangsu 214122, China;1. State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, People''s Republic of China;2. Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, People''s Republic of China;3. Shanghai Collaborative Innovation Center for Biomanufacturing, East China University of Science and Technology, Shanghai 200237, People''s Republic of China;1. Department of Chemical and Biochemical Engineering, University of Western Ontario, 1151 Richmond St., London, Ontario N6A 3K7, Canada;2. Sustainable Process Technology, Faculty of Science and Technology, University of Twente, P.O. Box 217, 7500 AE Enschede, Netherlands;3. Shell Global Solutions International, United States;4. RWTH Aachen, AVT – Biochemical Engineering, Worringerweg 1, 52074 Aachen, Germany;1. Department of Chemical and Biological Engineering, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA;2. Chemistry of Life Processes Institute, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA;3. Center for Synthetic Biology, Northwestern University, 2145 Sheridan Road, Evanston, IL 60208, USA;1. Centre for Synthetic Biology and Innovation, Imperial College London, London SW7 2AZ, UK;2. Department of Bioengineering, Imperial College London, London SW7 2AZ, UK
Abstract:
Keywords:Channeling  Free metabolites  Promoter  Reflux  Tryptophan  Violacein
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