Itaconic acid production from wheat chaff by Aspergillus terreus |
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| Institution: | 1. Department of Chemical Engineering, National Tsing Hua University, 101, Section 2, Kuang-Fu Road, 30013 Hsinchu, Taiwan;2. Material and Chemical Research Laboratories, Industrial Technology Research Institute, 101, Section 2, Kuang-Fu Road, 30013 Hsinchu, Taiwan;1. School of Food Science and Engineering, Hefei University of Technology, Hefei, Anhui Province 230009, PR China;2. China Rural Technology Development Center, Beijing 100045, PR China;3. College of Chemistry and Material Engineering, Chaohu University, Hefei, Anhui Province 238000, PR China;4. Key Laboratory for Agricultural Products Processing of Anhui Province, Hefei, Anhui Province 230009, PR China;1. iAMB – Institute of Applied Microbiology, ABBt – Aachen Biology and Biotechnology, RWTH Aachen University, Worringerweg 1, D-52074 Aachen, Germany;2. Department of Biology, Philipps-University Marburg, Karl-von-Frisch-Straße 8, D-35032 Marburg, Germany;3. LOEWE Center for Synthetic Microbiology (SYNMIKRO), D-35032 Marburg, Germany;4. Institut für Technische und Makromolekulare Chemie (ITMC), RWTH Aachen University, Worringerweg 2, D-52074 Aachen, Germany;5. BioSC, c/o Forschungszentrum Jülich, D-52425 Jülich, Germany;1. Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, Higashi-Hiroshima, Japan;2. Program of Biotechnology, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, Japan |
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| Abstract: | Biotechnologically produced itaconic acid is an important building block for the chemical industry and still based on pure carbon sources, detoxified molasses or starch hydrolysates. Changing these first generation feedstocks to alternative renewable resources of a second generation implies new challenges for the cultivation process of the industrial itaconic acid producer Aspergillus terreus, which is known to be very sensitive towards impurities. To select a suitable pretreatment method of a second generation feedstock, the influences of different hydrolysate components, like monosaccharides and sugar degradation products, were tested. Particular the impact of those components on itaconic acid yield, productivity, titer and morphology was investigated in detail. Wheat chaff was used as lignocellulosic biomass, which is an agricultural residue. An alkaline pretreatment method with sodium hydroxide at room temperature and a subsequent enzymatic saccharification at pH 4.8 at 50 °C with 10 FPU/gBiomass Biogazyme 2x proved to be very suitable for a subsequent biotechnological production of itaconic acid. A purification by a cation exchanger of the wheat chaff hydrolysate resulted in a final titer of 27.7 g/L itaconic acid with a yield of 0.41 g/gtotal sugar. |
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| Keywords: | Itaconic acid Lignocellulose Wheat chaff Alkaline pretreatment |
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