Biogenic synthesis of gold nanoparticles by marine endophytic fungus-Cladosporium cladosporioides isolated from seaweed and evaluation of their antioxidant and antimicrobial properties |
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| Affiliation: | 1. Department of Biochemistry, Mangalore University, Chikka Aluvara, Kodagu 571232, Karnataka, India;2. Department of Biochemistry, Mangalore University, Mangalagangotri, Mangalore 574199, Karnataka, India;3. Toxinology/Toxicology and Drug Discovery Unit, Centre for Emerging Technologies, Jain University, Ramanagara 562112, Karnataka, India;1. Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India;2. Department of Physics, Alagappa University, Karaikudi 630 004, India;3. Department of Zoology, University of Madras, Guindy Campus, Chennai 600 025, India;1. Department of Applied Biotechnology and Food Science, Budapest University of Technology and Economics, Budapest, Hungary;2. Institute for Technical Physics and Materials Science, Centre for Energy Research, Hungarian Academy of Sciences, Budapest, Hungary;3. Department of Physics, Budapest University of Technology and Economics, Budapest, Hungary;1. State Key Laboratory of Fine Chemicals, Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Environmental Science and Technology, Dalian University of Technology, Dalian 116024, China;2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;3. Key Laboratory of Environmental Biology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;1. Department of Microbiology, Kakatiya University, Warangal 506009, Telangana, India;2. University Arts and Science College Subedari, Hanamkonda, Warangal, India |
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| Abstract: | Marine endophytes are the most untapped group of microorganisms having enormous applications in pharmaceutical and cosmetra id="spar0060">Marine endophytes are the most untapped group of microorganisms having enormous applications in pharmaceutical and cosmetic industries. In the present study, we have optimized a method for biogenic synthesis of gold nanoparticles (AuNPs) from Cladosporium cladosporioides, an endophytic fungus of the seaweed, Sargassumwightii. The identity of the fungus was established by the 18 s rRNA and ITS sequence. The AuNPs synthesized using C. cladosporioides were characterized by UV–vis spectrophotometer, Field Emission Scanning Electron Microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, Dynamic light scattering, Atomic force microscopy, and Energy dispersive X-ray spectroscopic studies. They were tested for free radical scavenging activity (DPPH and FRAP assay) and antimicrobial activity against a panel of pathogenic microorganisms. The AuNps were within 100 nm as confirmed by the above methods. An attempt was made to understand the mechanism of the gold nanoparticle synthesis using the fungal extract. The present study shows the involvement of NADPH-dependent reductase and phenolic compounds in the bioreduction of the gold metal salts to nanoparticles. The AuNPs showed significant antioxidant as well as the antimicrobial activity. Hence, this study has shown a great potential for the development of a cost effective antimicrobial treatment utilizing biogenic gold nanoparticles. |
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| Keywords: | Gold nanoparticles Antimicrobial activity Marine endophytes |
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