Correlation of pigment production with mycelium morphology in extractive fermentation of Monascus anka GIM 3.592 |
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| Institution: | 1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China;2. Dongguan Tianyi Biotechnology Co. Ltd., Dongguan 523000, PR China;1. School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510006, PR China;2. School of Pharmacy, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China;3. School of Life Science and Biotechnology, and State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China;1. Jiangxi Key Laboratory of Bioprocess Engineering, Jiangxi Science and Technology Normal University, Nanchang, 330013, China;2. School of Life Science, Jiangxi Science and Technology Normal University, Nanchang, 330013, China;3. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, China;4. College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China;1. State Key Laboratory of Food Science and Technology, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China;2. Sino-German Joint Research Institute, Nanchang University, No. 235 Nanjing East Road, Nanchang, 330047, China;1. School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, PR China;2. State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, Shanghai 200240, PR China;3. School of Biological Science and Engineering, South China University of Technology, Guangzhou 510006, PR China;4. Department of Chemical Engineering, Pusan National University, Pusan 609-735, South Korea |
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| Abstract: | Extractive fermentation with nonionic surfactants is a potential method for producing Monascus pigments. In this study, the correlation between mycelium morphology and pigment production was investigated in extractive fermentation of Monascus anka GIM 3.592. The results demonstrated that pigment biosynthesis was associated with mycelial morphology and the accumulation of granular inclusions in cells. The physiological status in terms of hyphal and pellet diameters exhibited an excellent correlation with pigment accumulation, especially the yield of extracellular pigment in extractive fermentation (r > 0.85, p < 0.05). Nonionic surfactants could reduce pigment yield by influencing the morphology of hyphae and mycelium pellets. High yields of both intracellular and extracellular pigments could be achieved by controlling variations in hyphal diameters in two-stage extractive fermentation. Moreover, continuous extractive fermentation led to stable pigment production, with a relatively high productivity of total pigments reaching 72.3 AU/day. This study proposed a simple method for monitoring pigment biosynthesis in extractive fermentation using mycelium morphology as an indicating factor. |
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| Keywords: | Mycelium morphology Intracellular granular inclusions Metabolism control Extractive fermentation |
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