Combined Wharton’s jelly derived mesenchymal stem cells and nerve guidance conduit: A potential promising therapy for peripheral nerve injuries |
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| Institution: | 1. Medical Biochemistry Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;2. Histology & Cell Biology Department, Faculty of Medicine, Zagazig University, Zagazig, Egypt;3. Orthopedic Surgery Department, Faculty of Medicine, Benha University, Benha, Egypt;1. School of Medicine, Institute of Biomedicine, University of Eastern Finland, P.O. Box 1627, FIN-70211 Kuopio, Finland;2. Institute of Public Health and Clinical Nutrition, University of Eastern Finland, FIN-70211 Kuopio, Finland;1. Department of Clinical Science, Faculty of Veterinary Medicine, Urmia University, Nazloo Road, Urmia 57153 1177, Iran;2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Salmas Road, Urmia 3737, Iran;3. Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, 52 Campus Drive, Saskatoon, Saskatchewan S7N 5B4, Canada;1. Department of Biomedical Engineering, School of Basic Medical Sciences, Wuhan University, Wuhan 430071, China;2. Center of Molecular Medicine, School of Medicine, Hubei University of Arts and Sciences, Xiangyang 441053, China;3. Ingénierie Moléculaire et Physiopathologie Articulaire (IMoPA), UMR 7365 CNRS – Université de Lorraine, Biopôle, 54500 Vand?uvre-lès-Nancy, France |
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| Abstract: | BackgroundPeripheral nerve injuries represent a clinical problem with insufficient or unsatisfactory treatment options. Functional outcome with nerve guidance conduits was unsatisfactory in nerve defects with increased gap size. So, cell therapy may benefit as a tool for optimizing the regeneration process. The aim of the present study was to evaluate the impact of combination of cell therapy and nerve guidance conduits on the nerve regeneration and on the expression of the factors aiding the regeneration in a rat model of sciatic nerve injury.Methods and resultsSixty Wistar rats were randomly divided into four groups: Group I: normal control group; Group II: sciatic nerve injury (SNI) with a 10 mm long sciatic nerve gap; Group III: SNI with using a nerve conduit (NC) for nerve gap bridging; and Group IV: SNI with using a NC associated with Wharton’s jelly derived mesenchymal stem cells (WJ-MSCs). The results showed that the combination therapy NC + WJ-MSCs caused much better beneficial effects than NC alone evidenced by increasing sciatic nerve index and pin-prick score. The histopathological analysis found that the use of the NC combined with WJHYPHEN]MSCs resulted in a structure of the sciatic nerve comparable to the normal one with better nerve regeneration when compared with NC only. There was no differentiation of WJ-MSCs into nerve structure. Lastly, there was an upregulation of expression for netrin-1, ninjurin, BDNF, GDNF, VEGF and angiopoitin-1 rat genes in NC + WJ-MSCs group than NC alone.ConclusionThe addition of WJ-MSCs to the nerve guidance conduits seems to bring significant advantage for nerve regeneration, basically by increasing the expression of neurotrophic and angiogenic factors establishing more favorable environment for nerve regeneration. |
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| Keywords: | Peripheral nerve Nerve regeneration Nerve conduit Mesenchymal stem cells WJ-MSCs Neurotrophic factors Angiogenic factors |
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