Analysis of Brachypodium distachyon UVR8 reveals conservation in UV-B receptors |
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| Authors: | H. Chen Y. Yin J. Niu J. M. Kwak M. Du |
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| Affiliation: | 1. Shanxi Key Laboratory of Plant Macromolecules Stress Response, TaiYuan, P.R. China;2. Shanxi Key Laboratory of Plant Macromolecules Stress Response, TaiYuan, P.R. China School of Life Sciences, Shanxi Normal University, TaiYuan, P.R. China;3. Department of New Biology, DGIST, Daegu, Republic of Korea;4. School of Life Sciences, Shanxi Normal University, TaiYuan, P.R. China |
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| Abstract: | - The Ultraviolet Resistance Locus 8 (UVR8) in plants recognizes ultraviolet-B (UV-B) light and plays a crucial role in regulating plant growth through a series of signal transduction events. However, the UVR8 in monocotyledon crops has not yet been systematically analysed.
- We identified BdUVR8 (BRADI_3g45740) from the genome of Brachypodium distachyon, a relative of wheat, by analysing the phylogenetic tree, the gene expression pattern, detecting accumulation of UV-B response metabolites, and checking for phenotype recovery.
- The BdUVR8 protein sequence is similar to the known UVR8 of other species. The phylogenetic tree of UVR8 shows clear divergence between dicotyledons and monocotyledons. Expression analysis revealed that UV-B downregulates BdUVR8 by 70% and upregulates the chalcone synthase (BdCHS) gene 3.4-fold in B. distachyon. The pCAMBIA1300::BdUVR8-mCherry construct introduced into Arabidopsis uvr8 mutants showed that the BdUVR8 protein is localized in the cytoplasm and translocates into the nucleus in response to UV-B irradiation. The introduction of BdUVR8 into uvr8 rescued hypocotyl elongation caused by UV-B and restored expression of HY5, Chalcone synthase, and Flavanone 3-hydroxylase, as well as accumulation of total flavonoids.
- Together, our results show that BdUVR8 is a photoreceptor that perceives UV-B in B. distachyon.
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| Keywords: | Brachypodium distachyon ultraviolet resistance locus 8 UV radiation UV-B response |
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