Immunological effects of collagen and collagen peptide from blue shark cartilage on 6T-CEM cells |
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| Institution: | 1. Department of Food Science, Aarhus University, Blichers Allé 20, 8830 Tjele, Denmark;2. Department of Food Science, Faculty of Science, University of Copenhagen, Rolighedsvej 26, 1958 Frederiksberg C, Denmark;3. Department of Human Nutritional Sciences, University of Manitoba, Winnipeg, MB R3T 2N2, Canada;1. College of Food and Biological Engineering, Jimei University, Xiamen 361021, China;2. Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen 361021, China;3. Department of Food Science and Technology, Tokyo University of Marine Science and Technology, Tokyo 108-8477, Japan;4. Kokugakuin Tochigi Junior College, 601 Hirai-machi, Tochigi 328-8588, Japan |
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| Abstract: | The physicochemical and in vitro mechanism of immunologic tolerance of pepsin-soluble collagen and its peptide, CII-P, from blue shark cartilage were studied. Protein patterns showed three identical (α1)3 chains, suggesting that it was a type-II collagen (CII). CII-P had high antioxidant activity and low carbohydrate content. Collagens had better biocompatibility with decreased the viability of 6T-CEM cell compared to control cells (without collagen). Immunological indices such as FAS/APO-1, cytokine, and caspase levels were higher in CII-treated 6T-CEM cells. Collagen bound to 6T-CEM cell receptors in a dose-dependent manner, and an optimum effect was observed with 10 μg/mL collagen. The high carbohydrate content of CII could activate the FAS receptor, which led to increased apoptotic gene expression in 6T-CEM cells. Breakdown of 6T-CEM cell nuclei through the induction of apoptosis by CII was confirmed by fluorescence microscopy. Collagen molecular weight and glycosylation patterns were crucial factors for immunologic tolerance and 6T-CEM cellular apoptosis. |
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| Keywords: | Blue shark Collagen 6T-CEM cells Biocompatibility |
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