A flow-through enzymatic microreactor immobilizing lipase based on layer-by-layer method for biosynthetic process: Catalyzing the transesterification of soybean oil for fatty acid methyl ester production |
| |
| Affiliation: | 1. School of Pharmaceutical Science, Nanjing Tech University, Nanjing 211816, China;2. Biotechnology and Pharmarceutical Engineering, Nanjing Tech University, China;1. Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland;2. Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Toruń, Poland;1. Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Gagarina 7, 87-100 Toruń, Poland;2. Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University in Toruń, Wileńska 4, 87-100 Toruń, Poland;1. Computer Science and Engineering Department, University of Notre Dame, Notre Dame, IN 46556, USA;2. Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI 48109, USA;3. Department of Mechanical Engineering, Phayao University, Maeka, Muang, Phayao 56000, Thailand;4. Department of Mechanical and Aerospace Engineering, University of Texas Arlington, Arlington, TX 76019, USA;5. AT&T, 208 South Akard Street, Ste 110, Dallas, TX 75202, USA;6. Department of Industrial and Manufacturing Systems Engineering, University of Texas Arlington, Arlington, TX 76019, USA;7. TMAC, University of Texas Arlington, Arlington, TX 76019, USA;1. College of Bioengineering and Pharmaceutical Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China;2. College of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, Jiangsu 211816, China |
| |
| Abstract: | A simple and convenient method was proposed in this paper to develop a flow-through enzymatic micro-reactor made from polytetrafluoroethylene (PTFE). It consisted of the polydopamine layer (functioned as a primer) and layer by layer (LBL) coatings composed of polyethylenimine (PEI) and lipase. The multiple deposition of PEI and lipase was the key factor of increasing the enzyme loading on microreactor. After 8 PEI/lipase layers, enzyme loading on the inner surface of 5-m microchannel reached a maximum (350 μg to 400 μg), compared with approximately 20 μg in single layer. Microreactor with higher enzyme loading was successfully applied on transesterification of soybean oil for effective fatty acid methyl ester (FAME, biodiesel) production. A 95.2% conversion rate of biodiesel can be achieved in 53 min under optimized conditions, instead of a couple of hours in the traditional batch reaction. |
| |
| Keywords: | Immobilized enzyme microreactor Layer-by-layer assembly Biodiesel Polydopamine |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
