Enhanced selective oxidation of trimethylolpropane to 2,2-bis(hydroxymethyl)butyric acid using Corynebacterium sp. ATCC 21245 |
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| Affiliation: | 1. Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden;2. Department of Botany, Faculty of Science, South Valley University, Qena, Egypt;3. Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt;1. Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247 667, India;2. Department of Biotechnology, University of the Western Cape, Private Bag X17, Bellville 7530, South Africa;1. School of Chemical and Biological Engineering, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul, Republic of Korea;2. Interdisciplinary Program for Bioengineering, Seoul National University, Seoul, Republic of Korea;3. Department of Medical Biomaterials Engineering, Kangwon National University, 1 Kangwondaehak-gil, Chuncheon, Republic of Korea;1. Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India;2. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India;1. Laboratory of Biochemical Engineering, College of Pharmaceutical Engineering and Life Sciences, Changzhou University, Changzhou, China;2. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, China;3. Department of Bioengineering, Rice University, Houston, TX, USA |
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| Abstract: | 2,2-Bis(hydroxymethyl)butyric acid (BHMB) is an important multifunctional chemical for the emerging bio-based polymer industry. It can be produced from trimethylolpropane (TMP) by selective oxidation using growing cells of Corynebacterium sp. ATCC 21245. However, this process is limited by the low volumetric productivity and low concentration of the final product. In the present study, we performed sequential batch operation with cell recycling in media containing glycerol, acetic acid, and increasing concentrations of yeast extract. This approach enhanced the conversion of 10 and 15 g/L TMP to 11.0 and 16.3 g/L BHMB at rates of 0.50 and 0.20 g/L.h, respectively. Applying a cell bleeding strategy resulted in an overall 10-fold improvement in productivity. The consequently prolonged biocatalyst viability resulted in a quantitative conversion of 20 g/L TMP to 22.3 g/L BHMB and a yield of 1.10 gBHMB/gTMP (100% molar yield). This work facilitates further studies of the selective oxidation on other industrially important polyols. |
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| Keywords: | Selective oxidation Trimethylolpropane Sequential batch fermentation Cell recycling Cell bleeding 2,2-Bis(hydroxymethyl)butyric acid |
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