Tween 80 influences the production of intracellular lipase by Schizochytrium S31 in a stirred tank reactor |
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| Institution: | 1. Centre for Chemistry and Biotechnology, Deakin University, Waurn Ponds, Geelong 3217, Australia;2. Centre for Cellular and Molecular Biology, Council of Scientific and Industrial Research, Uppal Road, Hyderabad, Telangana 500007, India;1. Department of Microbiology and Biotechnology, Faculty of Natural Science, Vilnius University, 21/27, M. K. ?iurlionio Street, LT-03101, Vilnius, Lithuania;2. Department of Biochemistry and Molecular Biology, Faculty of Natural Science, Vilnius University, 21M.K. ?iurlionio Street, LT-03101, Vilnius, Lithuania;1. School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia;2. Centre of Excellence for Biomass Utilization, School of Bioprocess Engineering, Universiti Malaysia Perlis, 02600, Arau, Perlis, Malaysia;3. School of Chemical Engineering, Universiti Sains Malaysia, Engineering Campus, Pulau Pinang, Malaysia |
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| Abstract: | Marine microorganisms are a potential source of enzymes with structural stability, high activity at low temperature and unique substrate selectivity. Thraustochytrids are marine heterotrophic microbes, well known for the production of omega-3 fatty acids. In this study the effect of Tween 80 as a carbon source was investigated with regard to biomass, lipase and lipid productivity in Schizochytrium sp. S31. Tween 80 (1%) and 120 h of incubation were the optimum condition period for biomass, lipid and lipase productivity in a stirred tank reactor. The yields obtained were 0.9 g L?1 of biomass, 300 mg g?1 of lipid and 39 U/g of lipase activity. Sonication was optimised in terms of time and acoustic power to maximise the yield of extracted lipase. The extracted lipase from Schizochytrium S31 was observed to hydrolyse long chain polyunsaturated fatty acids DHA and EPA. |
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| Keywords: | Lipase DHA Omega-3 fatty acids Marine Bioactives |
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