Study of the interaction of human serum albumin with Alstonia scholaris leaf extract-mediated silver nanoparticles having bactericidal property |
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| Affiliation: | 1. Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, 721102, India;2. Chemical Biology Laboratory, Department of Sericulture, Raiganj University, Uttar Dinajpur, 733134, West Bengal, India;3. School of Medical Science and Technology, Indian Institute of Technology, Kharagpur, West Bengal, 721302, India;1. Department of Pharmaceutics, Himalayan Pharmacy Institute, Majhitar, Rangpo, East Sikkim, 737136, India;2. Department of Chemistry, University of North Bengal, Darjeeling, 734013, West Bengal, India;3. Department of Biotechnology, University of North Bengal, Darjeeling, 734013, West Bengal, India;4. Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India;5. Department of Chemistry, Tripura University, Agartala, 799022, Tripura, India;6. Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India;1. Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, 721102, West Bengal, India;2. Department of Microbiology, Vidyasagar University, Midnapore, 721102, West Bengal, India;3. Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, 721 102, West Bengal, India;4. Molecular and Applied Mycology and Plant Pathology Laboratory, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata 700019, West Bengal, India;5. Regional Plant Resource Centre, Bhubaneswar 751015, Odisha, India;1. State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, China;2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, Yunnan Province, China;3. The Third Affiliated Hospital of Kunming Medical University, Kunming 650106, Yunnan Province, China;1. Department of Biochemistry, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata 700054, India;2. Omics Laboratory, Department of Biotechnology, University of North Bengal, Siliguri 734013, West Bengal, India;1. Department of Microbiology, Vidyasagar University, Midnapore, West Bengal, India;2. Omics Laboratory, Department of Biotechnology, University of North Bengal, Siliguri, West Bengal, India;3. Department of Chemistry and Chemical Technology, Vidyasagar University, Midnapore, West Bengal, India;4. Immunology and Microbiology Laboratory, Department of Human Physiology with Community Health, Vidyasagar University, Midnapore, West Bengal, India |
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| Abstract: | This study investigates the green synthesis of AgNPs from 1 mM aqueous AgNO3 using 10% leaf extract of Alstonia scholaris (Chhatim) for its wide antibacterial and medicinal properties. The synthesized AgNPs were duly characterized by UV–vis (UV–vis) spectrophotometry, dynamic light scattering, field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive analysis of X-rays spectroscopy, and fourier transform infrared spectrophotometry. Their antibacterial property was tested against Escherichia coli (ATCC 25922), and minimum inhibitory concentrations of 0.08 nM of AgNPs were obtained, which suggests improved therapeutic efficacy. We report the interaction of human serum albumin (HSA) with this nanoparticle, and this interaction was studied by UV–vis, fluorescence, and circular dichroism spectroscopies and zeta potential measurement at room temperature. It was found that the AgNPs form a complex with HSA, which may cause the slightest change in the conformation of HSA. The calculated values of Stern-Volmer quenching constant, binding constant, and binding distance were 1.82 × 107 M−1, 1.58 × 107 M−1, and 3.68 nm, respectively. Therefore, in future, the present study may provide useful information to design a better antibacterial compound by using green synthesized nanoparticles with fewer side effects. |
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| Keywords: | AgNPs Characterization HSA Binding constant Binding distance |
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