Process engineering of cellulosic n-butanol production from corn-based biomass using Clostridium cellulovorans |
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| Affiliation: | 1. Department of Biomedical Engineering, University of Alabama at Birmingham (UAB), 1670 University Blvd., Birmingham, AL 35233, USA;2. Department of Chemical and Biological Engineering, The University of Alabama, 245 7th Avenue, Tuscaloosa, AL 35401, USA;3. Department of Chemical and Biomolecular Engineering, The Ohio State University, 151 West Woodruff Avenue, Columbus, OH 43210, USA;1. School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, UKM, 43600 Bangi, Selangor, Malaysia;2. Department of Chemical and Process Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, UKM, 43600 Bangi, Selangor, Malaysia;3. Department of Applied Microbiology, Faculty of Applied Sciences, Taiz University, 6803 Taiz, Yemen;4. Department of Chemical Engineering, Faculty of Chemical Engineering, Universiti Teknologi Malaysia, UTM, Skudai, 81310 Johor, Malaysia;1. CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China;2. University of Chinese Academy of Sciences, Beijing, China;1. Biosystems and Agricultural Engineering Department, Oklahoma State University, Stillwater, OK, USA;2. Department of Animal Sciences, The Ohio State University, and Ohio State Agricultural Research and Development Center, Wooster, OH, USA;3. Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA;1. Department of Chemical and Biological Engineering, The University of Alabama, 245 7th Avenue, Tuscaloosa, AL 35401, USA;2. Departments of Medicine and Biomedical Engineering, The University of Alabama at Birmingham, 703 19th Street South and 1530 3rd Avenue South, Birmingham, AL 35294, USA;3. William G Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 140 West 19th Avenue, Columbus, OH 43210, USA;1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, China;2. Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan;3. Research Center for Energy Technology and Strategy, National Cheng Kung University, Tainan 701, Taiwan |
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| Abstract: | The cellulolytic Clostridium cellulovorans has been engineered to produce n-butanol from low-value lignocellulosic biomass by consolidated bioprocessing (CBP). The objective of this study was to establish a robust cellulosic biobutanol production process using a metabolically engineered C. cellulovorans. First, various methods for the pretreatment of four different corn-based residues, including corn cob, corn husk, corn fiber, and corn bran, were investigated. The results showed that better cell growth and a higher concentration of n-butanol were produced from corn cob that was pretreated with sodium hydroxide. Second, the effects of different carbon sources (glucose, cellulose and corn cob), basal media and culture pH values on butanol production were evaluated in the fermentations performed in 2-L bioreactors to identify the optimal CBP conditions. Finally, the engineered C. cellulovorans produced butanol with final concentration >3 g/L, yield >0.14 g/g, and selectivity >3 g/g from pretreated corn cob at pH 6.5 in CBP. This study showed that the fermentation process engineering of C. cellulovorans enabled a high butanol production directly from agricultural residues. |
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| Keywords: | Butanol fermentation Consolidated bioprocessing Corn residues |
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