Ultrasound-assisted production of highly-purified β-glucan schizophyllan and characterization of its immune properties |
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| Institution: | 1. Contipro a.s., Dolní Dobrouč 401, 561 02 Dolní Dobrouč, Czech Republic;2. University of Pardubice, Faculty of Chemical Technology, Department of General and Inorganic Chemistry, Studentska 573, 532 10 Pardubice 2, Czech Republic;3. Department of Experimental Biology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic;4. 3rd Medical Faculty, Charles University in Prague, Ruska 2411/87, 100 00 Prague, Czech Republic;1. Glyn O. Phillips Hydrocolloid Research Centre at HUT, School of Food and Pharmaceutical Engineering, Faculty of Light Industry, Hubei University of Technology, Wuhan 430068, China;2. Glyn O. Phillips Hydrocolloid Research Centre, Glyndwr University, Plas Coch, Mold Road, Wrexham LL11 2AW, UK;1. Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran;2. Microbiology Department, Faculty of Biological Sciences, Alzahra University, 1993891176 Tehran, Iran;3. Division of Glycoscience, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Centre, SE-10691 Stockholm, Sweden;1. Division of Applied Chemistry and Biochemistry, National Institute of Technology, Tomakomai College, Nishikioka 443, Tomakomai, Hokkaido 059 1275, Japan;2. Itochu Sugar Co. Ltd, Tamatsuura 3, Hekinan, Aichi 447 8506, Japan;3. Division of Biotechnology and Macromolecular Chemistry, Graduate School of Chemical Sciences and Engineering, Faculty of Engineering, Hokkaido University, Sapporo, Hokkaido 060 8628, Japan;4. Department of Chemistry and Biochemistry, National Institute of Technology, Fukushima College, Nagao 30, Iwaki, Fukushima 970 8034, Japan;1. Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran;2. Division of Glycoscience, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Centre, SE-10691 Stockholm, Sweden;3. Microbiology Department, Faculty of Biological Sciences, Alzahra University, 19938-91176 Tehran, Iran;1. Hebei Normal University of Science and Technology, Qinhuangdao, Hebei 066600, China;2. School of Chinese Medicine, Hong Kong Baptist University, Hong Kong, China;3. Food Science and Technology Program, Beijing Normal University-Hong Kong Baptist University United International College, Zhuhai, Guangdong 519085, China |
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| Abstract: | In this work, we suggest procedure for manufacturing of highly pure schizophyllan (SPG) cleaved into shorter fragments by ultrasonication. The ultrasonicated SPG (uSPG) was prepared from Schizophyllum commune submerged cultivation in bioreactor, culture broth ultrasonication with 100 Ws/mL energy input, followed by depth filtration and ultrafiltration. The uSPG contained less than 0.3%w/w protein and 0.078 IU/mg endotoxin. Chemical composition and structure of the uSPG were analyzed by GC and NMR, and conformation of the molecules was documented by AFM. Apyrogenicity of uSPG was proved by MAT test. Immunomodulatory properties of native and denatured uSPG were studied in vitro with human whole blood. The LPS-spiked samples revealed immunomodulatory activity of both native and denatured uSPG. Protein array showed that native uSPG activates certain cytokines and chemokines significantly stronger than denatured uSPG. |
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| Keywords: | Schizophyllan ultrasound Downstream Proteins Endotoxins Immune effect |
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