Microbial community dynamics in aerated biological fluidized bed (ABFB) with continuously increased p-nitrophenol loads |
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| Affiliation: | 1. Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden;2. Department of Botany, Faculty of Science, South Valley University, Qena, Egypt;3. Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt;1. Department of Chemistry, Aligarh Muslim University, Aligarh 202002, India;2. Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh 202002, India;1. Departamento de Biologia Molecular e Celular, Universidade Federal da Paraíba, João Pessoa, PB, Brazil;2. Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, Diadema, SP, Brazil;3. Departamento de Bioquímica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil;4. Departamento de Biofísica, Escola Paulista de Medicina, Universidade Federal de São Paulo, São Paulo, SP, Brazil;5. Departamento de fitotecnia, Universidade Federal da Paraíba, Centro de Ciências Agrárias, Areia, PB, Brazil;6. Instituto Agronômico de Campinas, Campinas, SP, Brazil;1. Applied Biological Sciences Program, Chulabhorn Graduate Institute, Bangkok 10210, Thailand;2. Laboratory of Biotechnology, Chulabhorn Research Institute, Bangkok 10210, Thailand;3. Center of Excellence on Environmental Health and Toxicology, Ministry of Education, Bangkok 10400, Thailand |
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| Abstract: | Biodegradability of PNP has been reported widely in recent years, but the community composition of PNP-degrading microorganisms was still unclear today. In this paper, the biodegradation process with continuously PNP loading from 0 to 6.50 kg m−3 d−1 in 58 days in an aerobic biological fluidized bed (ABFB) reactor has been investigated. The results show that COD and PNP removal stabilized at 95% and 99% during the operation period with a maximum PNP concentration of 1250 mg/L. The high concentration of PNP in substrate led to a significant increase in extracellular polymeric substances (EPS) component of biomass and obvious morphological changes of microbial colonies during the degradation process. In addition, high-throughput sequencing was employed to reveal the highly diverse bacterial and fungal populations in the reactor. At the same time, genera Sphingobium, Penicillum and Debaryomyces belonging to phyla Proteobacteria and Ascomycota were identified to be the dominant species in high concentration PNP degradation process. This work investigated the tolerable degree of aerobic microbes to PNP toxicity as well as the characteristics of microbial communities at different PNP concentration levels. It might add some new insights into bacterial and fungal communities in high p-nitrophenol concentration degradation processes. |
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| Keywords: | Microbial community High-throughput sequencing Aerated biological fluidized bed |
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