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Combining Gal4p-mediated expression enhancement and directed evolution of isoprene synthase to improve isoprene production in Saccharomyces cerevisiae
Institution:1. Institute of Bioengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, PR China;2. Key Laboratory of Biomass Chemical Engineering of Ministry of Education, Zhejiang University, Hangzhou 310027, PR China;1. Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, St Lucia, QLD, Australia;2. Commonwealth Research Organization (CSIRO), Land and Water, Ecosciences Precinct, 41 Boggo Road, Dutton Park 4102, Queensland, Australia;3. Department of Chemistry and Biomolecular Sciences, Macquarie University, NSW, Australia;4. Amyris, Emeryville, CA, USA;1. VTT Technical Research Centre of Finland Ltd., P.O. Box 1000, FI-02044 VTT, Espoo, Finland;2. Global Technology, SK Innovation, Daejeon, Republic of Korea;1. Key Laboratory of Systems Bioengineering (Ministry of Education), Tianjin University, Tianjin 300072, PR China;2. SynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, PR China;1. Department of Biopharmaceutical Sciences, Synthetic Biology Engineering Lab of Henan Province, School of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, PR China;2. Changdu Bureau of Science and Technology, Changdu 854000, PR China;1. Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, St. Lucia, QLD 4072, Australia;2. Metabolomics Australia (Queensland Node), The University of Queensland, St. Lucia, QLD 4072, Australia;1. Laboratório Nacional de Energia e Geologia, I.P., Unidade de Bioenergia, Estrada do Paço do Lumiar 22, 1649-038 Lisboa, Portugal;2. Department of Biotechnology and Physical Chemistry, Faculty of Chemical Engineering and Technology, Cracow University of Technology, Warszawska 24, 31-155 Cracow, Poland
Abstract:
Keywords:Isoprene  Directed evolution  Isoprene synthase
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