Enhancing erythritol productivity in Yarrowia lipolytica using metabolic engineering |
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| Affiliation: | 1. Unité de Biotechnologies et Bioprocédés, Université Libre de Bruxelles, Belgium;2. Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège - Gembloux Agro-Bio Tech, Belgium;3. Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, 78350 Jouy-en-Josas, France;1. School of Biotechnology, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China;2. College of Life and Environmental Sciences, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, China;3. Shanghai Laiyi Center for Biopharmaceutical R&D, 800 Dongchuan Road, Shanghai 200240, China;4. George Stevens Academy, 23 Union St, Blue Hill, ME 04614, USA;5. Shanghai Institute of Pharmaceutical Industry, 1320 West Beijing Road, Shanghai 200040, China;6. Key Laboratory of Synthetic Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, 300 Fenglin Road, Shanghai 200032, China;7. Shanghai Research and Development Center of Industrial Biotechnology, 528 Ruiqing Road, Shanghai 201201, China;8. Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM), 200 North Zhongshan Road, Nanjing 211816, China;1. Jiangsu Key Laboratory for Biomass-Based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China;2. Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China;3. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China;1. State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Jinan 250100, China;2. School of Life Science, Linyi University, Linyi 276000, China;3. School of Energy and Environment, City University of Hong Kong, Tat Chee Avenue, Kowloon, Hong Kong SAR;1. Jiangsu Key Laboratory for Biomass-based Energy and Enzyme Technology, Huaiyin Normal University, Huaian, China;2. Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration, Huaiyin Institute of Technology, Huaian, China;3. Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China;1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, PR China;2. Shanghai Collaborative Innovation Center for Biomanufacturing Technology (SCICBT), 130 Meilong Road, Shanghai 200237, PR China;1. State Key Laboratory of Microbial Metabolism, and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China;2. College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing, China;3. School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China;4. Microbial Processes and Interactions, TERRA Teaching and Research Centre, University of Liège- Gembloux Agro-Bio Tech, Belgium |
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| Abstract: | Erythritol (1,2,3,4-butanetetrol) is a four-carbon sugar alcohol with sweetening properties that is used by the agrofood industry as a food additive. In this study, we demonstrated that metabolic engineering can be used to improve the production of erythritol from glycerol in the yeast Yarrowia lipolytica. The best results were obtained using a mutant that overexpressed GUT1 and TKL1, which encode a glycerol kinase and a transketolase, respectively, and in which EYK1, which encodes erythrulose kinase, was disrupted; the latter enzyme is involved in an early step of erythritol catabolism. In this strain, erythritol productivity was 75% higher than in the wild type; furthermore, the culturing time needed to achieve maximum concentration was reduced by 40%. An additional advantage is that the strain was unable to consume the erythritol it had created, further increasing the process's efficiency. The erythritol productivity values we obtained here are among the highest reported thus far. |
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| Keywords: | Erythritol Metabolic engineering Glycerol kinase Transketolase Erythrulose kinase |
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