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A genome-wide phylogeny and the diversification of genus Liriomyza (Diptera: Agromyzidae) inferred from anchored phylogenomics |
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| Authors: | Jing-Li Xuan Sonja J. Scheffer Owen Lonsdale Brian K. Cassel Matthew L. Lewis Charles S. Eiseman Wan-Xue Liu Brian M. Wiegmann |
| Affiliation: | 1. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA;2. Systematic Entomology Laboratory, USDA, Agricultural Research Service, Beltsville, Maryland, USA Contribution: Conceptualization (equal), Data curation (lead), Funding acquisition (lead), Investigation (equal), Methodology (equal), Project administration (equal), Resources (lead), Supervision (equal), Validation (equal), Writing - review & editing (equal);3. Agriculture and Agri-Food Canada, Ottawa, Ontario, Canada Contribution: Data curation (lead), Investigation (equal), Resources (equal), Validation (equal), Writing - review & editing (equal);4. Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA Contribution: Data curation (lead), Investigation (equal), Methodology (equal), Validation (equal);5. Systematic Entomology Laboratory, USDA, Agricultural Research Service, Beltsville, Maryland, USA Contribution: Data curation (equal), Investigation (equal), Resources (equal), Validation (equal);6. 276 Old Wendell Rd., Northfield, Massachusetts, USA Contribution: Resources (equal), Validation (equal), Writing - review & editing (equal);7. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, PR China Contribution: Resources (equal), Writing - review & editing (equal) |
| Abstract: | The genus Liriomyza Mik (Diptera: Agromyzidae) is a diverse and globally distributed group of acalyptrate flies. Phylogenetic relationships among Liriomyza species have remained incompletely investigated and have never been fully addressed using molecular data. Here, we reconstruct the phylogeny of the genus Liriomyza using various phylogenetic methods (maximum likelihood, Bayesian inference, and gene tree coalescence) on target-capture-based phylogenomic datasets (nucleotides and amino acids) obtained from anchored hybrid enrichment (AHE). We have recovered tree topologies that are nearly congruent across all data types and methods, and individual clade support is strong across all phylogenetic analyses. Moreover, defined morphological species groups and clades are well-supported in our best estimates of the molecular phylogeny. Liriomyza violivora (Spencer) is a sister group to all remaining sampled Liriomyza species, and the well-known polyphagous vegetable pests [L. huidobrensis (Blanchard), L. langei Frick, L. bryoniae. (Kaltenbach), L. trifolii (Burgess), L. sativae Blanchard, and L. brassicae (Riley)]. belong to multiple clades that are not particularly closely related on the trees. Often, closely related Liriomyza species feed on distantly related host plants. We reject the hypothesis that cophylogenetic processes between Liriomyza species and their host plants drive diversification in this genus. Instead, Liriomyza exhibits a widespread pattern of major host shifts across plant taxa. Our new phylogenetic estimate for Liriomyza species provides considerable new information on the evolution of host-use patterns in this genus. In addition, it provides a framework for further study of the morphology, ecology, and diversification of these important flies. |
| Keywords: | anchored hybrid enrichment host use Liriomyza morphology phylogeny |