Kinetics of cellulose hydrolysis by halostable cellulase from a marine Aspergillus niger at different salinities |
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| Affiliation: | 1. Biotechnology, Department of Chemistry, Center for Chemistry and Chemical Engineering, Lund University, SE-22100 Lund, Sweden;2. Department of Botany, Faculty of Science, South Valley University, Qena, Egypt;3. Department of Microbiology and Immunology, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt;1. School of Chemistry, Physics and Mechanical Engineering, Science and Engineering Faculty, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, Australia;2. Geology Department, School of Mines, Federal University of Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, MG 35400-00, Brazil |
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| Abstract: | Kinetics of cellulose hydrolysis with halostable cellulase from a marine Aspergillus niger was analyzed at different salinities. Cellulase activity in 8% NaCl solution was 1.43 folds higher than that in NaCl free solution. Half saturation constant, Km (15.6260 g/L) and the rate constant of deactivation, Kde (0.3369 g/L h) in 8% NaCl solution was lower than that (18.6364 g/L), 0.3754 (g/L h) in NaCl free solution. The maximum initial hydrolysis velocity, Vmax (25.5295 g/L h), in 8% NaCl solution was higher than that in NaCl free solution (25.0153 g/L h). High salinity increased affinity to the cellulase to the substrate and thermostability. Halostable cellulase from a marine Aspergillus niger was valuable for cellulose hydrolysis under high salinity conditions. |
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| Keywords: | Halostable cellulase Hydrolysis kinetics High salinity |
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