Validation of single nucleotide polymorphism quantification in pooled DNA samples with SNaPIT. A glycosylase-mediated methods for polymorphism detection method |
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Authors: | Curran Sarah Hill Linzy O'Grady Geraldine Turic Dragana Asherson Philip Taylor Eric Sham Pak Craig Ian Vaughan Pat |
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Institution: | (1) S.G.D.P Research Centre, Institute of Psychiatry, De Crespigny Park, Denmark Hill, Box Pobo, SE5 8AF London, UK |
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Abstract: | Association studies using genome scans to identify quantitative trait loci for multifactorial disorders, with anything approaching
reasonable power, have been compromised by the need for a very dense array of genetic markers and large numbers of affected
individuals. These requirements impose enormous burdens on the genotyping capacity for most laboratories. DNA pooling has
been proposed as a possible approach to reduce genotyping costs and effort. We report on the application of the SNaPIT™ technology
to evaluate allele frequencies in pooled DNA samples and conclude that it offers a cost effective, efficient and accurate
estimator and provides several advantages over competing technologies in this regard. |
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Keywords: | Allele frequency genotyping glycosidase single nucleotide polymorphisms SNaPIT technology |
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