Effect of submerged culture conditions on exopolysaccharides production by Armillaria luteo-virens Sacc QH and kinetic modeling |
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Authors: | De Qin Xu Ming Liang Fu Qi He Chen Jing Liu |
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Institution: | (1) College of Chemistry and Biology Science, Lishui University, Lishui, 323000, China;(2) Department of Food Science and Nutrition, Zhejiang University, Kaixuan Rd. 268, Hangzhou, 310029, People’s Republic of China; |
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Abstract: | This work aimed to develop the submerged cultivation conditions for improved exopolysaccharides (EPS) production by Armillaria luteo-virens Sacc. The effects of culture temperature, aeration rate, inoculum level, initial pH, and additives on EPS formation and mycelial
growth are investigated. The aeration rate, initial pH, and inoculum level significantly affected EPS production under the
submerged cultivation. The developed conditions were as follows: cultivation temperature 23 °C, initial pH 5.0, aeration rate
0.5 vvm, 0.5% Tween 80, inoculum level 5% (v/v), and shaking speed 120 r/min. Under the developed conditions, the highest
EPS production was 13.01 g/L at 5 days culture time. EPS production was examined in a 5 L bioreactor, and an unstructured
kinetic model for EPS formation was well developed. The verified investigations in the large-scale cultivation system showed
that the developed models are able to predict the submerged cultivation process of EPS formation. Current results revealed
that the submerged cultivation conditions can be utilized to control EPS production, and the unstructured models developed
are suitable for explaining EPS production by A. luteo-virens Sacc QH in a large-scale cultivation bioreactor. |
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