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Identification of low-abundance proteins via fractionation of the urine proteome with weak anion exchange chromatography
Authors:Chih-Ming Lu  Yu-Jen Wu  Cheng-Chi Chen  Jue-Liang Hsu  Jiing-Chuan Chen  Jeff Yi-Fu Chen  Chun-Hsiung Huang  Ying-Chin Ko
Institution:(1) Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;(2) Department of Urology, Buddhist Da Lin Tzu Chi General Hospital, Chiayi, Taiwan;(3) Department of Beauty Science, Meiho University, Pingtung, Taiwan;(4) Graduate Institute of Biotechnology, National Pingtung University of Science and Technology, Pingtung, Taiwan;(5) Department of Food Science and Nutrition, Meiho University, Pingtung, Taiwan;(6) Department of Biotechnology, Kaohsiung Medical University, Kaohsiung, Taiwan;(7) Department of Urology, Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan;(8) Center of Excellence for Environmental Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
Abstract:

Background  

Low-abundance proteins are difficultly observed on the two-dimensional gel electrophoresis (2-DE) maps of urine proteome, because they are usually obscured by high-abundance proteins such as albumin and immunoglobulin. In this study, a novel fractionation method was developed for enriching low-abundance proteins by removing high-abundance proteins and progressive elution with salts of various concentrations.
Keywords:
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