首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Histone H2A.Z acid patch residues required for deposition and function
Authors:Kurt Jensen  Maria Soledad Santisteban  Craig Urekar  M Mitchell Smith
Institution:(1) Department of Microbiology, University of Virginia Health System, University of Virginia, P.O. Box 800734, Charlottesville, VA 22908-0734, USA;(2) Present address: Department of Neurology, McKnight Brain Institute, University of Florida, 100 S. Newell Dr., P.O. Box 100236, Gainesville, FL 32610-0236, USA;(3) Present address: Department of Biology, University of North Carolina at Pembroke, One University Drive, P.O. Box 1510, Pembroke, NC 28372-1510, USA
Abstract:The incorporation of histone variants is one mechanism used by the eukaryotic cell to alter the generally repressive chromatin template. However, the exact molecular mechanisms that direct this incorporation are not well understood. The SWR1 chromatin remodeling complex that binds to and directs incorporation of histone variant H2A.Z into chromatin has been characterized, but significantly less information is available concerning the requirements on the H2A.Z target molecule. We performed an unbiased mutagenic screen designed to elucidate the function of H2A.Z in Saccharomyces cerevisiae. The screen identified residues within the conserved acidic patch of H2A.Z as being important for the function of the variant. We characterized single point mutations in the patch that are phenotypically sensitive to a variety of growth conditions and are expressed at lower protein levels, but are functionally defective (htz1-D99A, htz1-D99K, and htz1-E101K). The mutants were significantly less detectable by chromatin immunoprecipitation at PHO5, a gene previously described to be enriched for H2A.Z. These results identify acidic patch residues of H2A.Z that are critical for mediating deposition and function in chromatin, and represent potential candidates for the interaction of H2A.Z with its deposition and/or targeting machinery.
Keywords:
本文献已被 PubMed SpringerLink 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号