Process performance of parallel bioreactors for batch cultivation of Streptomyces tendae |
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Authors: | Ralf Hortsch Harald Krispin Dirk Weuster-Botz |
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Institution: | (1) Lehrstuhl f?r Bioverfahrenstechnik, Technische Universit?t M?nchen, Boltzmannstr. 15, 85748 Garching, Germany; |
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Abstract: | Batch cultivations of the nikkomycin Z producer Streptomyces tendae were performed in three different parallel bioreactor systems (milliliter-scale stirred-tank reactors, shake flasks and shaken
microtiter plate) in comparison to a standard liter-scale stirred-tank reactor as reference. Similar dry cell weight concentrations
were measured as function of process time in stirred-tank reactors and shake flasks, whereas only poor growth was observed
in the shaken microtiter plate. In contrast, the nikkomycin Z production differed significantly between the stirred and shaken
bioreactors. The measured product concentrations and product formation kinetics were almost the same in the stirred-tank bioreactors
of different scale. Much less nikkomycin Z was formed in the shake flasks and MTP cultivations, most probably due to oxygen
limitations. To investigate the non-Newtonian shear-thinning behavior of the culture broth in small-scale bioreactors, a new
and simple method was applied to estimate the rheological behavior. The apparent viscosities were found to be very similar
in the stirred-tank bioreactors, whereas the apparent viscosity was up to two times increased in the shake flask cultivations
due to a lower average shear rate of this reactor system. These data illustrate that different engineering characteristics
of parallel bioreactors applied for process development can have major implications for scale-up of bioprocesses with non-Newtonian
viscous culture broths. |
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