Differential pulse polarographic determination of plasma menadione |
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Authors: | Steven A. Akman Fumiyo Kusu Kiyoko Takamura Rowan Chlebowski Jerome Block |
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Affiliation: | 1. Division of Medical Oncology, Harbor-UCLA Medical Center, Torrance, California, 90509, USA;2. Tokyo College of Pharmacy, Tokyo, Japan |
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Abstract: | A differential pulse polarographic assay for plasma vitamin K3 (menadione) has been developed. Details of the assay are (i) lipid-soluble material is extracted from plasma into ether by the method of Bjornsson et al. [(1978) Thromb. Haemostas.2, 466–473]; (ii) ether is evaporated under nitrogen and the residue is dissolved in the supporting electrolyte, methanol: 0.2 m borate buffer (9:1), pH 6.8; (iii) current height is measured at ?0.32 V vs SCE on the differential pulse polarogram. The lower sensitivity limit of this technique after addition of standard vitamin K3 to plasma is 0.3 μm; the calibration curve is linear from 0.6 through 10 μm. Two patients treated with a single dose of menadiol sodium diphosphate, 20 mg/M2 i.m., achieved measurable plasma vitamin K3 levels at 0.5 to 1.0 h ranging between 0.5 (0.08 μg/ml) and 2 μm (0.3 μg/ml). |
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Keywords: | polarography voltammetry electrochemical vitamin K menadione quinone |
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