A method of purification of partially methylated alditol acetates in the methylation analysis of glycoproteins and glycopeptides |
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Authors: | Mark E Lowe Bo Nilsson |
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Institution: | 1. Pediatric Metabolism Branch, NIADDK National Institutes of Health, Bethesda, Maryland 20205 USA;2. Laboratory of Pathology, NCI, National Institutes of Health, Bethesda, Maryland 20205 USA |
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Abstract: | A method for methylation analysis of intact glycoproteins is described. Starting with intact glycoprotein, the oligosaccharides are methylated, hydrolyzed, reduced, and acetylated. The partially methylated alditol acetates are then separated from noncarbohydrate contaminants on a silica gel G column. Partially methylated hexitol acetates are eluted from the column with petroleum ether:ethyl acetate (1:1, ) and partially methylated N-acetylhexosaminitol acetates are subsequently eluted with methanol. Analysis by gas-liquid chromatography/mass spectrometry of the partially methylated alditol acetates shows no interfering contaminants. This method circumvents the need to make pronase glycopeptides and avoids the pitfalls of other methylation procedures. |
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Keywords: | glycoprotein methylation silica glycopeptide oligosaccharide |
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