Quantitation of the delivery of liposome contents into plant protoplasts |
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| Authors: | Adrian J. Cutler F. Constabel W.G.W. Kurz Peter D. Shargool |
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| Affiliation: | 1. Department of Biochemistry, University of Saskatchewan, Saskatoon S7N 0W0, Canada;2. Plant Biotechnology Institute, National Research Council of Canada, Saskatoon S7N 0W9, Canada |
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| Abstract: | A procedure for the quantitation of the delivery of liposome contents into Catharanthus roseus protoplasts has been developed. The method is based on the uptake of liposome encapsulated methylumbelliferyl β-d-glucoside and its enzymatic hydrolysis to yield fluorescent methylumbelliferone. Since the free glucoside is not taken up by the protoplasts to a significant extent, the delivery of material in the nanomole range can be measured with ease. |
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| Keywords: | liposome plant protoplast glucosidase To whom correspondence should be addressed. |
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