Determination of choline in erythrocytes using high-resolution proton nuclear magnetic resonance spectroscopy: Comparison with a choline oxidase method |
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Authors: | Georg R. Beilharz Carl R. Middlehurst Philip W. Kuchel Glenn E. Hunt Gordon F.S. Johnson |
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Affiliation: | 1. Department of Biochemistry, University of Sydney, Sydney, New South Wales 2006, Australia;2. Department of Psychiatry, University of Sydney, Sydney, New South Wales 2006, Australia |
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Abstract: | Detailed operating conditions are reported for the determination of choline in human erythrocytes using proton nuclear magnetic resonance spectroscopy in conjunction with the inversion-recovery spin-echo pulse sequence. The results of the NMR method were in excellent agreement with those obtained using an enzymatic (choline oxidase) assay; however, they were approximately three times higher than those reported using gas chromatography/mass spectrometry techniques. The differences may be partly due to the method of preparing or sampling cells since there is a distribution of choline in cells of different ages. However, choline levels were not affected by the methods used in the present study for storing or preparing cells. |
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Keywords: | choline blood NMR analysis method assay |
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