Accurate and efficient method for quantification of urinary histamine by gas chromatography negative ion chemical ionization mass spectrometry |
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Authors: | LJackson Roberts John A Oates |
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Institution: | 1. Department of Pharmacology, Vanderbilt University, Nashville, Tennessee 37232 USA;2. Department of Medicine, Vanderbilt University, Nashville, Tennessee 37232 USA |
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Abstract: | Because of the recognized inaccuracy and unreliability of currently available methods for the quantification of histamine in biological fluids, a method for quantification of urinary histamine by stable isotope dilution assay with negative ion chemical ionization mass spectrometry has been developed. Following the addition of 2H4]histamine to 1 ml of urine, histamine is extracted into butanol, back-extracted into HCl, derivatized to the pentafluorobenzyl derivative (CH2C6F5)3-histamine, extracted into methylene chloride, and then quantified with negative ion chemical ionization mass spectrometry by selected ion monitoring of the ratio of ions . Twenty samples can be assayed in 2 days. Precision of the assay is ±2.7% and the accuracy is 97.6%. Lower limits of sensitivity are approximately 100–500 fg injected on-column. This assay provides a very sensitive, accurate, and efficient method for the quantification of histamine in human urine. |
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Keywords: | histamine mass spectrometry negative ion chemical ionization gas chromatography urinary |
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