A competitive displacement assay to detect saxitoxin and tetrodotoxin |
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| Authors: | Stephen R. Davio Paulito A. Fontelo |
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| Affiliation: | Pathophysiology Division, U. S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland 21701 USA |
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| Abstract: | An assay is described which detects saxitoxin (STX) and tetrodotoxin (TTX) by their competitive displacement of [3H]saxitoxin from its receptor in rat brain membranes. The assay has a sensitivity of 0.15 ng STX/ml and 0.8 ng TTX/ml for buffer samples. The assay was also applied to detection of these toxins in unextracted human plasma and found to have a sensitivity of 0.5 ng STX/ml and 0.6 ng TTX/ml. The competitive displacement assay appears to be the most sensitive procedure yet for detection of STX and TTX. |
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| Keywords: | saxitoxin tetrodotoxin seafood toxins neurotoxins binding assay competitive displacement assay |
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