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Nicotinamide: a natural inhibitor of tRNA methylase
Authors:R M Halpern  S Q Chaney  B C Halpern  R A Smith
Affiliation:1. Department of Biochemistry, Ludwig-Maximilians-Universität München, Munich, Germany;2. Gene Center, Ludwig-Maximilians-Universität München, Munich, Germany;3. Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore, USA;4. Max Planck Institute of Biochemistry, Martinsried, Germany;5. King’s College London School of Cancer & Pharmaceutical Sciences, London, UK;6. Technical University of Munich, TUM School of Natural Sciences, Department of Bioscience, Garching, Germany;7. Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, USA;1. MOE Key Laboratory of Biosystems Homeostasis & Protection, Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology, and Innovation Center for Cell Signaling Network, Life Sciences Institute, Zhejiang University, Hangzhou 310058, China;2. Cancer Center, Zhejiang University, Hangzhou 310058, China;3. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China;4. MOE Key Laboratory of Model Animal for Disease Study and State Key Laboratory of Pharmaceutical Biotechnology, Model Animal Research Center, Nanjing University, Nanjing 210061, China;5. Department of Hepatobiliary and Pancreatic Surgery, Zhejiang Provincial Key Laboratory of Pancreatic Disease, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310058, China;6. Institute of Aging Research, Hangzhou Normal University, Hangzhou 311121, China;7. Center for Life Sciences, Shaoxing Institute, Zhejiang University, Shaoxing 321000, China;1. CanmetMATERIALS, Natural Resources Canada, Hamilton, ON L8P 0A5, Canada;2. Department of Mechanical, Automotive, and Materials Engineering, University of Windsor, Windsor, ON N9B 3P4, Canada;3. Tempel Steel Co., Chicago, IL 60640-1020, USA
Abstract:A dialyzable tRNA methylase inhibitor has been isolated from rat liver. By chromatographic and electrophoretic data as well as ultraviolet and mass spectral data, the inhibitor is identified as nicotinamide. Kinetic evidence indicates that nicotinamide is an inhibitor of tRNA methylase.
Keywords:
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