Enzymatic preparation of [1-13C]d-fructose-6-phosphate from [13C]formaldehyde and d-ribose-5-phosphate using the formaldehyde-fixing system of Methylomonas aminofaciens 77a |
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| Authors: | Hideshi Yanase Koji Matsuzaki Yusuke Sato Keiko Kita Yoshiyuki Sato Nobuo Kato |
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| Institution: | (1) Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, 606 Kyoto, Japan;(2) Present address: Institute for Fundamental Research, Suntory Ltd., Mishima-gun, 618 Osaka, Japan |
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| Abstract: | The intracellular concentration of 5,8,11,14,17-cis-eicosapentaenoic acid (EPA) and 4,7,10,13,16,19-cis-docosahexaenoic acid (DHA) was carried out by Mortierella alpina 1S-4 in a medium containing fish oil as the main carbon source. The EPA and DHA contents reached 29.2% and 20.0% of total fatty acids, respectively, when the fungus was grown in a medium containing salmon oil (EPA and DHA contents, 14.0% and 17.3%, respectively) as the main carbon source in a 5-1 bench-scale fermentor. EPA and DHA in the added fish oil were incorporated into both the mycelial polar lipids and triglycerides.On leave from Suntory Ltd.
Correspondence to: S. Shimizu |
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