Isolation and Partial Characterization of Antifungal Metabolites Produced by <Emphasis Type="Italic">Bacillus</Emphasis> sp. IBA 33 |
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Authors: | María C Maldonado Jose Corona María A Gordillo Antonio R Navarro |
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Institution: | 1.Instituto de Biotecnología, Facultad de Bioquímica Química y Farmacia,Universidad Nacional de Tucumán,Tucumán,Argentina |
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Abstract: | Antifungal proteins produced by Bacillus sp. IBA 33 were purified by ammonium sulfate precipitation and DEAE-Sephacel column chromatography. The two purified proteins
inhibited the growth of Geotrichum candidum, the sour rot disease agent in lemon. The proteins were stable at 20 (3 months), 40, 60 and 100°C (30 min) and remained active
after sterilization at 121°C for 15 min. Their hydrophobic nature was proved and when were developed with ninhydrin they did
not show any free amino groups. The infrared spectrum showed vibrational modes corresponding to peptide, ester or ketone links
and saturated CH links corresponding to long chain fatty acids. UV scan spectroscopy showed tyrosine and or tryptophan amino
acids in their composition. The remarkable thermo-resistance of proteins may be a good feature to be used in the development
of a new biocontrol method of Geotrichum candidum. |
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