Biosorption of metal ions byAzotobacter vinelandii |
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Authors: | D Cotoras M Millar P Viedma J Pimentel A Mestre |
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Institution: | (1) Departamento de Bioquimica y Biologia Molecular, Facultad de Ciencias Quimicas y Farmacéuticas, Universidad de Chile, Casilla 233, Santiago 1, Chile |
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Abstract: | Azotobacter vinelandii was better than eitherDerxia gummosa orRhizobium trifolii for sorption of UO
2
2+
. Its maximum binding capacity was 0.25 mmol UO
2
2+
/g dry biomass with an affinity constant of 333 l/mmol at pH 4.1 according to the Langmuir model. In a semisynthetic medium,A. vinelandii showed the highest sorption capacity in the early stationary phase. The binding of UO
2
2+
, Cu2+, Ca2+ and Zn2+ was affected by the pH of the solution. With HCl as eluent, virtually all the sorbed UO
2
2+
was released. The presence of Cu2+, Cd2+, Ca2+, and Zn2+ inhibited the UO
2
2+
biosorption whereas Mg2+ and K+ had no effect. |
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Keywords: | Azotobacter vinelandii biosorption cadmium copper metals polysaccharide uptake uranium zinc |
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