Genotypic variability for protoplast regeneration in Saintpaulia ionantha (H. Wendl.) |
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Authors: | Traud Winkelmann Jürgen Grunewaldt |
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Affiliation: | (1) Institute for Ornamental Plant Breeding, Bornkampsweg 31, D-22926 Ahrensberg, Germany;(2) Present address: Dept. of Floriculture, Institute for Vegetable and Ornamental Crops, Mittelhäuser Str., D-99189 Kühnhausen, Germany |
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Abstract: | Summary The behaviour of eleven Saintpaulia ionantha (H. Wendl.) genotypes in protoplast culture was compared. Isolation of protoplasts from young shootlets regenerated in vitro on leaf explants, yielded 0.7 to 1.8 × 106 protoplasts per gram fresh weight. In all cultivars and breeding lines tested, cell divisions were observed. The mean division frequencies varied between 1.0 and 5.0% after 14 days, and between 6.4 and 13.8% after 24 days of culture. In ten genotypes callussing and shoot regeneration were achieved. The difference between the genotypes in shoot regeneration rate, between 2 and 68%, was more pronounced. The comparison of four cytokinins indicated hat thidiazuron was most effective for shoot regeneration, but often resulted in poorer shoot quality than benzylaminopurine.Abbreviations BAP (6-Benzylaminopurine) - IAA (In-dole-3-acetic acid) - NAA (-Naphthaleneacetic acid) - TDZ (Thidiazuron = 1-Phenyl-3-(1,2,3-thiadiazol-5-yl)-urea) - 2iP (6-(,-dimethylallylamino)-purine) |
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Keywords: | Protoplast regeneration Saintpaulia ionantha Genotypic differences |
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